Research

Membrane proteins (MPs) are key mediators for physiologically essential cell–cell and cell–environment communications. These proteins thus have received intensive fundamental and translational research focus, as evidenced by the fact that half of the currently marketed pharmaceuticals target MPs. Our researches have mainly focused on the ligand discovery and tool development for MPs. We have designed and synthesized a plethora of tool ligands for the chemical biology and medicinal studies over a range of MPs, mainly pharmaceutically important G protein-coupled receptors (GPCRs). The boost in the structural studies of GPCRs in the past decade greatly facilitated the design of tool ligands for many purposes, including the compounds for structural biology, fluorescence labeling, nuclear magnetic resonance (NMR) and electronic microscope (EM). We also developed a series of conceptually and structurally novel detergents, which facilitated the solubilization and stabilization of MPs and innovated the biophysics and biochemical studies.

bride

Ligand discovery for GPCRs

More
bride

Tool ligand development

More
bride

Detergents and other amphiphiles

More

Lab Members

Tao Houchao 陶厚朝

Ph.D, Princpial Investigator, 2014–

Zhao Fei 赵飞

Ph.D, RA, 2014-

Hu Tao 胡涛

Doctoral Student, 2016-

Xie Linshan 谢林杉

Doctoral Student, 2018-

Liu Lu 刘路

Graduate Student, 2018-

Li Cuixia 李翠霞

Graduate Student, 2019-

Yang Meifang 杨梅芳

Graduate Student, 2019-

Qiu Yanli 邱艳丽

Graduate Student, 2020-

Luo Weiling 罗卫灵

Graduate Student, 2020-

Former Lab Members

Wu Dong 武栋

Associate Professor, 2014-2021

Wang Wei 王伟

Alumni, 2015-2016

Lu Wanglong 卢望龙

Alumni, 2016-2017

Li Tao 李涛

Research Associate, 2015-2017

Zhang Wei 张威

Assistant Professor, 2018-2019

Zhu Zhihao 朱志豪

Visiting Student, 2018-2020

Xue Dongxiang 薛东香

Alumni, 2014–2020

Zhou Fang 周方

Alumni, 2015–2020

Publications

Yang D, Zhou Q, Labroska V, Qin S, Darbalaei S, Wu Y, Yuliantie E, Xie L, Tao H, Cheng J, Liu Q, Zhao S, Shui W, Jiang Y, Wang MW. G protein-coupled receptors: structure- and function-based drug discovery. Signal Transduct Target Ther. 2021 Jan 8;6(1):7.

Chen X, Xu Y, Qu L, Wu L, Han GW, Guo Y, Wu Y, Zhou Q, Sun Q, Chu C, Yang J, Yang L, Wang Q, Yuan S, Wang L, Hu T, Tao H, Sun Y, Song Y, Hu L, Liu ZJ, Stevens RC, Zhao S, Wu D, Zhong G. Molecular Mechanism for Ligand Recognition and Subtype Selectivity of α2C Adrenergic Receptor. Cell Rep. 2019 Dec 3;29(10):2936-2943.e4.

Xue D, Xu T, Wang H, Wu M, Yuan Y, Wang W, Tan Q, Zhao F, Zhou F, Hu T, Jiang Z, Liu ZJ, Zhao S, Liu D, Wüthrich K, Tao H. Disulfide-Containing Detergents (DCDs) for the Structural Biology of Membrane Proteins. Chemistry. 2019 Sep 6;25(50):11635-11640.

Xu Y, Wang Y, Wang Y, Liu K, Peng Y, Yao D, Tao H, Liu H, Song G. Mutagenesis facilitated crystallization of GLP-1R. IUCrJ. 2019 Oct 17;6(Pt 6):996-1006.

Xue D, Ye L, Zheng J, Wu Y, Zhang X, Xu Y, Li T, Stevens RC, Xu F, Zhuang M, Zhao S, Zhao F, Tao H. The structure-based traceless specific fluorescence labeling of the smoothened receptor. Org Biomol Chem. 2019 Jun 26;17(25):6136-6142.

Xue D, Wang J, Song X, Wang W, Hu T, Ye L, Liu Y, Zhou Q, Zhou F, Jiang ZX, Liu ZJ, Tao H. A Chemical Strategy for Amphiphile Replacement in Membrane Protein Research. Langmuir. 2019 Mar 26;35(12):4319-4327.

Zhou F, Ding K, Zhou Y, Liu Y, Liu X, Zhao F, Wu Y, Zhang X, Tan Q, Xu F, Tan W, Xiao Y, Zhao S, Tao H. Colocalization Strategy Unveils an Underside Binding Site in the Transmembrane Domain of Smoothened Receptor. J Med Chem. 2019 Nov 14;62(21):9983-9989.

Zhang X, Zhao F, Wu Y, Yang J, Han GW, Zhao S, Ishchenko A, Ye L, Lin X, Ding K, Dharmarajan V, Griffin PR, Gati C, Nelson G, Hunter MS, Hanson MA, Cherezov V, Stevens RC, Tan W, Tao H, Xu F. Crystal structure of a multi-domain human smoothened receptor in complex with a super stabilizing ligand. Nat Commun. 2017 May 17;8:15383.

Ye L, Ding K, Zhao F, Liu X, Wu Y, Liu Y, Xue D, Zhou F, Zhang X, Stevens RC, Xu F, Zhao S, Tao H. A structurally guided dissection-then-evolution strategy for ligand optimization of smoothened receptor. Medchemcomm. 2017 Apr 28;8(6):1332-1336.

Welcome to our lab!
Tel:021-20685016
E-mail: taohc@shanghaitech.edu.cn;
Address: Room B309, Ren Building, 230 Haike Road,
Shanghai,China 201210